Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: PINK1 contained in huMSC-derived exosomes prevents cardiomyocyte mitochondrial calcium overload in sepsis via recovery of mitochondrial Ca2+ efflux

Fig. 3

Assessment of cardiomyocyte mitochondrial Ca2+ (mCa2+) uptake, efflux, and the corresponding regulator. ac mCa2+ uptake and efflux in isolated permeabilized ACMs; dig.: digitonin, thaps.: thapsigargin, Ru360: MCU inhibitor, CGP: NCLX inhibitor; the box shows a magnified mCa2+ efflux tracing (260 s–360 s); R indicates the ratio of the ratiometric reporter Fura-2 (340/380 nm excitation and 510 nm emission). R/R0 indicates the ratio at each time point over the ratio at time 0. d mCa2+ efflux rate, n = 3 replicates per group; **p < 0.01, #p < 0.05. e FQ-PCR detected the mRNA expression levels of Mcu, Micu1, and Nclx in the heart after CLP for 12 h. f Western blotting was used to detect MCU, MICU1, and NCLX expression in hearts 12 h after CLP. GAPDH was used as a loading control

Back to article page