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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Heat shock preconditioning mesenchymal stem cells attenuate acute lung injury via reducing NLRP3 inflammasome activation in macrophages

Fig. 3

HS-pretreated UC-MSCs modulate the phenotypic polarization and secretion function of alveolar macrophages. The surface markers CD206 (a) and TNF-α (b) of alveolar macrophages from BALF were detected by flow cytometry analysis. The data are presented as the mean ± SEM (n = 5 mice/group). c The levels of TNF-α, IL-1β, and IL-6 in BALF from each group were measured by ELISA. The data are presented as the mean ± SEM (n = 5 mice/group). Flow cytometry analyses of CD206 (d) and TNF-α (e) expression in macrophages in each group. f The mRNA expression of TNF-α, IL-1β, and IL-6 in THP-1 cells co-cultured with each group of MSCs. g The mRNA expression of TNF-α, IL-1β, and IL-6 in THP-1 cells treated with conditioned medium from each group of MSCs. The data are presented as the mean ± SEM (n = 3/group). *p < 0.05, **p < 0.01, ***p < 0.001 (all p values were obtained by one-way ANOVA)

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Stem Cell Research & Therapy

ISSN: 1757-6512