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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Heat shock preconditioning mesenchymal stem cells attenuate acute lung injury via reducing NLRP3 inflammasome activation in macrophages

Fig. 5

HS pretreatment enhanced the secretion abilities of UC-MSCs by increasing HSP70 expression. a The levels of IL-10, TGF-β, and PGE2 in UC-MSC culture supernatants from each group were measured by ELISA. The data are presented as the mean ± SEM (n = 3/group). b Protein levels of HSP70 in UC-MSCs after knockdown of HSP70 were detected by immunoblotting. The immunoblotting results were quantified by analyzing the gray values. The data are expressed as the mean ± SEM (n = 3/group). c Flow cytometry analyses of TNF-α and CD206 expression in macrophages of each group. The data are presented as the mean ± SEM (n = 3/group). d Protein levels of NLRP3, ASC, pro-Caspase 1, cleaved-Caspase 1, and IL-1β in macrophages were detected by immunoblotting. The immunoblotting results were quantified by analyzing the gray values. The data are expressed as the mean ± SEM (n = 3/group). *p < 0.05, **p < 0.01, ***p < 0.001 (all p values were obtained by one-way ANOVA)

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Stem Cell Research & Therapy

ISSN: 1757-6512