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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Role of CD44 in increasing the potency of mesenchymal stem cell extracellular vesicles by hyaluronic acid in severe pneumonia

Fig. 3

Effect of Different Molecular Weights of Hyaluronic Acid Primed MSC EV on PA103 Bacterial Phagocytosis. a, b Extracellular bacterial CFU levels in the culture medium and Intracellular bacterial CFU levels as measured in LPS stimulated monocytes when treated with different MW HA primed MSC EV. Administration of HMW HA (1.0 MDa) primed MSC EV reduced extracellular PA103 CFU and increased intracellular PA103 CFU levels when compared with MSC EV or LMW HA (40 KDa) primed MSC EV or HMW HA (1.0 MDa) alone. c, d Intracellular bacterial CFU levels of LPS stimulated monocytes when treated with CD44 siRNA pretreated MSC EV with or without HMW HA. CD44 siRNA pretreatment of MSC EV prior to HMW HA incubation decreased bacterial phagocytosis by human monocytes compared to negative control (NC) siRNA pretreated MSC EV. Data are mean ± SD, **P < 0.01, ***P < 0.001, ****P < 0.0001 by ANOVA (Bonferroni), N = 3. Individual P values describe the statistical comparison between the two groups by Student’s t test. A dose of MSC EV (90 μl) was used = 2.0 × 1010 particles

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