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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Human bone marrow-derived, pooled, allogeneic mesenchymal stromal cells manufactured from multiple donors at different times show comparable biological functions in vitro, and in vivo to repair limb ischemia

Fig. 1

Gene expression profiling. a Unsupervised hierarchical clustering/condition tree of samples, HFF-1, three batches of Stempeucel®-1 and two batches of Stempeucel®-1A together based on the similarity of their expression profiles. It is evident from the condition tree based on the distance that out of 24,498 genes, an average of ≥ 77% of the global profiles of all the Stempeucel® products is similar compared to the HFF cells indicating a passage-independent similarity in global gene expression profiles. ~ 20% of the genes (4924 out of 24,498 genes) analyzed were differentially expressed between Stempeucel®-1 and Stempeucel®-1A. In total, ~ 3% of the genes (722 out of 24,498 genes analyzed) were significantly differentially expressed with a fold change of ≥ 2. b Analysis of genes expressed by Stempeucel®-1 (X-axis) and Stempeucel®-1A (Y-axis) with a significantly high correlation of R2 = 0.9827 in a scatter plot. c Comparative evaluation of twenty-three angiogenic genes in HFF-1 cell line, three batches of Stempeucel®-1, and two batches of Stempeucel®-1A. d Real-time PCR analysis of five selected angiogenic genes co-expressed across the Stempeucel® batches compared to HFF-1; VEGF—*P = 0.03, AGPT1—*P = 0.04, TGF b1—*P = 0.01, HGF—P = 0.03, and CXCL8—*P = 0.02. e Comparative evaluation of thirty-four anti-inflammation genes in HFF-1, three batches of Stempeucel®-1 and two batches of Stempeucel®-1A. f Real-time PCR analysis of five selected anti-inflammatory genes co-expressed across Stempeucel® batches compared to HFF-1; IDO—**P = 0.001, IL-10—**P = 0.004, VCAM1 **P = 0.002, PDL1—**P = 0.009, and HLA-G—**P = 0.002

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