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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Oxidant therapy improves adipogenic differentiation of adipose-derived stem cells in human wound healing

Fig. 6

NCT-treatment attenuates the anti-adipogenic effects of M(IFNG/LPS) macrophages. a Representative microscopy images of day 14 differentiated adipocytes exposed to macrophage-CM during the differentiation. Macrophages were either co-treated with PBS or NCT (500 μM) during their activation period. b Flow cytometry analysis of day 14 differentiated adipocytes cultured in the presence of macrophage-CM during differentiation. Values depict relative numbers of large lipid containing adipocytes compared to Mo-CM-treated cells, n=6. c Representative immunoblots analyzing expression levels of the adipocyte marker proteins FASN, ACC, PLIN1, and FABP4 in day 14 differentiated cells. d IL1B mRNA expression in macrophages co-treated with or without NCT during their activation. Data were normalized to the 18S rRNA reference gene and shown as mean –ΔCt, n=4. e Flow cytometry analysis of adipocytes differentiated in the presence of macrophage CM for 14 days supplemented with IL1B-inhibitor IL1RA or PBS (ctr). Values depict relative numbers of large lipid containing adipocytes compared to Mo-CM-treated cells, n=3. Asterisks indicate p values < 0.05 (*), <0.01 (**), and <0.001 (***), respectively. Statistical significance was determined by using non parametric unpaired Student’s t test. Data are shown as mean ± SEM

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