Table 1 Human primer sequences using quantitative real time polymerase chain reaction
Genes | Primer sequences | Tm | |
|---|---|---|---|
Adipsin | Forward | 5′-GGGCAGCGTGTACTTATCCT-3′ | 55 |
Reverse | 5′- AGAACCCCAAGATGCACAAC -3′ | ||
PPAR-γ | Forward | 5′- GCGGCTACTACAACCAGAGC -3′ | 55 |
Reverse | 5′- GCACATGGCACGTGTATCTC -3′ | ||
Adiponectin | Forward | 5′-GAGCTGACGTGGAAGATGAG-3′ | 55 |
Reverse | 5′-CTTCAAGTGCTGTCTGATTCCAATG-3′ | ||
Leptin | Forward | 5′-ATGCTGCAAACTGACCACGC-3′ | 55 |
Reverse | 5′-GCTTCGCTTTGCCAATGCTT-3′ | ||
LPL | Forward | 5′-TGAGTTTGCAGAAGTTTCCA-3′ | 60 |
Reverse | 5′-CCTTTGCCTCAGCATAGTTT-3′ | ||
FABP4 | Forward | 5′-GCATGGCCAAACCTAACATGA-3′ | 55 |
Reverse | 5′-CCTGGCCCAGTATGAAGGAAA-3′ | ||
C/EBPα | Forward | 5′-TGTATACCCCTGGTGGGAGA-3′ | 60 |
Reverse | 5′-TCATAACTCCGGTCCCTCTG-3′ | ||
C/EBPβ | Forward | 5′-CTTCAGCCCGTACCTGGAG-3′ | 60 |
Reverse | 5′-GGAGAAGGAAGTCGTGGTGC-3′ | ||
TSHR | Forward | 5′-GACACTGAAGCTGTACAACAATGG-3′ | 60 |
Reverse | 5′-AGACACGTCCAGCAAGCTTGGT-3′ | ||
SREBP2 | Forward | 5′-CAAGGCCCTGGAAGTGACAGA-3′ | 60 |
Reverse | 5′-AGGAACTCTGCTGCCCATCTG-3′ | ||
HMGCR | Forward | 5′-GCCTGGCTCGAAACATCTGAA-3′ | 60 |
Reverse | 5′-CTGACCTGGACTGGAAACGGATA-3′ | ||
ICAM-1 | Forward | 5′-CAGTCACCTATGGCAACGACTC-3′ | 60 |
Reverse | 5′-CTCTGGCTTCGTCAGAATCAC-3′ | ||
IL-1β | Forward | 5′-ATGAGTGCTCCTTCCAGGA-3′ | 60 |
Reverse | 5′-GATAGGTTCTTCAAAGATG-3′ | ||
TNF-α | Forward | 5′-CCAGAGGGAAGAGTTCCCCA-3′ | 55 |
Reverse | 5′-TCAGCTTGAGGGTTTGCTACAAC-3′ | ||
IL-6 | Forward | 5′-TCCACAAGCGCCTTCGGTCCAGTTG-3′ | 55 |
Reverse | 5′-AGAGGTGAGTGGCTGTCTGTGTGGG-3′ | ||
TGF-β1 | Forward | 5′-TACCAGAAATACAGCAACAATTCC-3′ | 55 |
Reverse | 5′-AAAGCCCTCAATTTCCCCTCC-3′ | ||
TGF-β2 | Forward | 5′-TGGTGAAAGCAGAGTTCAGAG-3′ | 55 |
Reverse | 5′-CACAACTTTGCTGTCGATGTAG-3′ | ||
GAPDH | Forward | 5′-TCCTTCTGCATCCTGTCAGCA-3′ | 60 |
Reverse | 5′-CAGGAGATGGCCACTGCCGCA-3′ |