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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: MiR-34a suppression targets Nampt to ameliorate bone marrow mesenchymal stem cell senescence by regulating NAD+-Sirt1 pathway

Fig. 2

MiR-34a overexpression exacerbates senescence-associated variations of young P3MSCs. a Morphological features and EGFP expression under fluorescence microscope (scale bar = 50 μm). Demonstration of the transduction efficiency of miR-34a overexpression in P3MSCs by RT-qPCR. Quantitative analysis for cell surface area and cell aspect ratio in miR-NC and miR-34a over-expressed P3MSCs. b Cell growth curves in miR-34a over-expressed P3MSCs. c Cell population doubling time (PDT). d Detection of cell cycle by flow cytometry and analysis of proliferation index (PI) and S-phases fraction (SPF). e Determination of osteogenic differentiation in miR-34a over-expressed P3MSCs by Alizarin Red S staining (scale bar = 100 μm). f SA-β-gal staining (scale bar = 50 μm) and quantification of β-gal positive cell numbers. g Gene expression of the senescence-related factors p16INK4a and p21WAF1/CIP ; n = 3 independent experiments. *P < 0.05, **P < 0.01 vs. miR-NC, N.S., not significant

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