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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: MiR-34a suppression targets Nampt to ameliorate bone marrow mesenchymal stem cell senescence by regulating NAD+-Sirt1 pathway

Fig. 3

MiR-34a suppression alleviates MSC replicative senescence in P10MSCs. a Morphological features and the presence of EGFP-expressing P10MSCs (scale bar = 50 μm). Evaluation of the transduction efficacy of miR-34a depletion in P10MSCs by RT-qPCR. Quantification for cell surface area and cell aspect ratio in anti-NC and anti-34a expressed P10MSCs. b Logarithmic proliferation in miR-34a-deficient P10MSCs. c Population doubling time (PDT). d Assessment of cell cycle. e Observation of osteogenesis (scale bar = 100 μm) and quantification. f Cellular SA-β-gal staining (scale bar = 50 μm) and quantification. g Detection of mRNA expression levels of senescence markers p16INK4a and p21WAF1/CIP in replicative senescent P10MSCs after miR-34a suppression by RT-qPCR; n = 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. P10 anti-NC, N.S., not significant

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