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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: MiR-34a suppression targets Nampt to ameliorate bone marrow mesenchymal stem cell senescence by regulating NAD+-Sirt1 pathway

Fig. 5

Measurement of Nampt expression and NAD+-Sirt1 signaling in replicative and natural senescence. a Determination of Nampt mRNA expression levels by RT-qPCR. b Determination of Nampt protein expression levels by western blot. ce Determination of basal NAD+ level (c), NAD+/NADH ratio (d), and Sirt1 deacetylase activity in P3MSCs, P10MSCs, and OMSCs, respectively; n = 3 independent experiments. **P < 0.01 vs. P3MSCs

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