Fig. 6

MiR-34a directly targets Nampt. a Schematic representation of the predicted complimentary base pairing between miR-34a and the 3′UTR of Nampt and the mutated binding site of putative miR-34a seed sequence. b Analysis of luciferase activity in cells co-transfected with miR-NC or miR-34a and plasmid containing the 3′ UTR of the wild type (WT) Nampt or a mutated (Mut) Nampt sequence in luciferase reporter assay. c Detection of Nampt protein expression levels by western blotting. d Detection of Nampt mRNA expression levels by RT-qPCR after interfering with miR-34a expression; n = 3 independent experiments. **P < 0.01 vs. miR-NC, P10 anti-NC, or O anti-NC