Fig. 1
From: A dual cardiomyocyte reporter model derived from human pluripotent stem cells
Generation of aMHC-mCherry H9 reporter line. a Diagram of the proposed strategy for reporter line derivation. Construct expressing aMHC promoter-driven mCherry and blasticidin-resistant (BSDr) genes was designed and delivered into hPSCs via lentivirus, followed by drug selection and single-cell colony derivation. b Representative brightfield (BF) and mCherry images of live aMHC-mCherry H9 cells. c PCR genotyping of hPSC clones after drug selection is shown. Forward and reverse primers were designed within mCherry coding sequencing, and wild-type (WT) H9 cells were used as a negative control. d Representative immunostaining and flow cytometry results of NANOG, OCT4, and SSEA4 are shown. e Representative immunostaining images of SOX17 and PAX6 for endodermal and ectodermal differentiation, respectively, were shown. Scale bars, 100 μm