Fig. 1
From: AQP0 is a novel surface marker for deciphering abnormal erythropoiesis
Microarray analysis of sorted erythroid differentiation stages for human and mouse bone marrow. Microarray analysis of sorted erythroid differentiation stages from human bone marrow (a). Human bone marrow cells were isolated and stained with anti-CD45 antibodies conjugated with PerCP, anti-CD71 antibodies conjugated with PE, and anti-CD235a antibodies conjugated with FITC. A portion of CD45high cells was sorted as nonerythroid cells. CD45−/dim/SSClow cells were gated and analyzed further. CD71high/CD235alow and CD71high/CD235ahigh were defined as indicating the early stage and late stage of erythropoiesis, respectively. The numbers 3913 and 4377 indicate the genes that are specifically expressed in the early and late erythroid differentiation stages relative to nonerythroid cells. The number 4580 indicates the gene that is specifically expressed in the late erythroid differentiation stage relative to the early erythroid differentiation stage. The numbers 1751, 3404, and 1726 indicate the gene shared with each pair (early/nonerythroid vs. late/nonerythroid, late/nonerythroid vs. late/early, and late/early vs. early/nonerythroid). The number 1110 indicates the gene shared with all three groups (early/nonerythroid, late/nonerythroid, and late/early). Microarray analysis of sorted erythroid differentiation stages from mouse bone marrow (b). Mouse bone marrow cells were isolated and stained with anti-CD71 antibodies conjugated with FITC and anti-TER119 antibodies conjugated with APC. A portion of CD71−/dim/TER119−/dim cells was sorted as nonerythroid cells. CD71high/TER119low and CD71high/TER119high were defined as indicating the early stage and late stage of erythropoiesis, respectively. The numbers 1430 and 4060 indicate the genes that are specifically expressed in early and late erythroid differentiation stages relative to nonerythroid cells. The number 4567 indicates the gene that is specifically expressed in the late erythroid differentiation stage relative to the early erythroid differentiation stage. The numbers 819, 3709, and 788 indicate the genes shared with each pair (early/nonerythroid vs. late/nonerythroid, late/nonerythroid vs. late/early, and late/early vs. early/nonerythroid). The number 711 indicates the gene that is shared by all three groups (early/nonerythroid, late/nonerythroid, and late/early)