Fig. 2

Characterization of mouse erythropoiesis stages using three surface markers (CD71, TER119, and Aqp0). Flow cytometry analysis of mouse erythropoiesis (a). Mouse bone marrow cells were isolated and stained with anti-CD71 antibodies conjugated with FITC, anti-TER119 antibodies conjugated with PE/Cy7, and anti-Aqp0 antibodies conjugated with Alexa Fluor 647. The percentages of positive cells of each antibody in unstained control were showed on the top. CD71^−/dim/TER119^−/dim cells were gated as nonerythroid cells. CD71^high/TER119^low, CD71^high/TER119^high, CD71^dim/TER119^high, and CD71⁻/TER119^high were defined as region 1 (R1), region 2 (R2), region 3 (R3), and region 4 (R4), respectively. R3 was gated, and the relationship between FSC (cell size) and Aqp0 expression was analyzed. The percentages of Aqp0⁺ cells in each population were analyzed and quantified (b). Data are representative of four independent experiments and reported as the mean ± standard deviation