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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: AQP0 is a novel surface marker for deciphering abnormal erythropoiesis

Fig. 4

Characterization of human erythropoiesis stages using three surface markers (CD71, CD235a, and AQP0). Flow cytometry analysis of human erythropoiesis (a). Human bone marrow cells were isolated and stained with anti-CD71 antibodies conjugated with PE/Cy7, anti-CD235a antibodies conjugated with FITC, and anti-AQP0 antibodies conjugated with Alexa Fluor 647. The percentages of positive cells of each antibody in unstained control were showed on the top. CD71−/dim/CD235a−/dim cells were gated as nonerythroid cells. CD71high/CD235alow, CD71high/CD235ahigh, CD71dim/CD235ahigh, and CD71−/CD235ahigh were defined as region 1 (R1), region 2 (R2), region 3 (R3), and region 4 (R4), respectively. R3 was gated, and the relationship between FSC (cell size) and AQP0 expression was analyzed. The percentages of AQP0high cells in each population were analyzed and quantified (b). The percentages of AQP0low and AQP0high cells in R3 were shown (c). The data are representative of three independent experiments and reported as the mean ± standard deviation

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