Fig. 5

Characterization of erythropoiesis of patients using three surface markers (CD71, CD235a, and AQP0). Clinical complete blood count values of nine patients (a). The bone marrow cells from nine patients were isolated and stained with anti-CD71 antibodies conjugated with PE/Cy7, anti-CD235a antibodies conjugated with FITC, and anti-AQP0 antibodies conjugated with Alexa Fluor 647. CD71^−/dim/CD235a^−/dim cells were gated as nonerythroid cells. CD71^high/CD235a^low, CD71^high/CD235a^high, CD71^dim/CD235a^high, and CD71⁻/CD235a^high were defined as region 1 (R1), region 2 (R2), region 3 (R3), and region 4 (R4), respectively. R3 was gated, and the relationship between FSC (cell size) and AQP0 expression was analyzed. The percentages of AQP0^high cells in each population were analyzed and quantified in patient 1 (b), patient 2 (c), patient 3 (d), patient 4 (e), patient 5 (f), patient 6 (g), patient 7 (h), patient 8 (i), and patient 9 (j). The data are representative of one independent experiment