Fig. 2

hERG deficiency did not affect the differentiation of hESC-CMs. a Schematic illustration of hESCs in vitro differentiation protocols using small molecule-based methods. b The hERG expression assessed by western blotting analysis. c Immunofluorescence staining of cardiomyogenic differentiation markers TNNT2 and α-actinin. Scale bar = 25 μm. d, e Flow cytometry analyses of CMs marker TNNT2 expression at days 15 without purification. f, g Immunostaining for the protein expression of MLC2v and MLC2a in WT and KOs. Scale bar = 25 μm. Data are expressed as means ± S.E.M. of 3 independent experiments