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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: The role of Serpina3n in the reversal effect of ATRA on dexamethasone-inhibited osteogenic differentiation in mesenchymal stem cells

Fig. 1

Effects of ATRA on osteogenic differentiation in MEFs. a ALP staining showed the effect of different concentrations of ATRA on ALP activities in MEFs after 5 and 7 days. b Quantification of ALP staining demonstrated the effect of different concentrations of ATRA on ALP activities in MEFs after 5 and 7 days, *p < 0.05 vs DMSO, **p < 0.01 vs DMSO. c Alizarin Red S staining showed the effect of different concentrations of ATRA on matrix mineralization in MEFs after 21 days. d Quantification of matrix mineralization in MEFs after 21 days, *p < 0.05 vs DMSO, **p < 0.01 vs DMSO. e MEFs infected with adenovirus-mediated Ad-GFP and Ad-BMP9 after 24 h. f Western blot demonstrated the protein expression of BMP9 in MEFs 24 h after Ad-GFP and Ad-BMP9 infection. g ALP staining showed the effect of different concentrations of ATRA on BMP9-induced ALP activities in MEFs after 5 and 7 days. h Quantification of ALP staining showed the effect of different concentrations of ATRA on BMP9-induced ALP activities in MEFs after 5 and 7 days, *p < 0.05 vs BMP9 + DMSO, **p < 0.01 vs BMP9 + DMSO. i RTqPCR showed the effect of different concentrations of ATRA on BMP9-induced osteogenic markers after 24 h, *p < 0.05 vs BMP9 + DMSO, **p < 0.01 vs BMP9 + DMSO. j Western blot results showed the effect of different concentrations of ATRA on BMP9-induced osteogenic markers after 24 h (numbers between the bands were the ratios of target bands to GAPDH using ImageJ). k Microscopic view of Alizarin Red S staining showed the effect of different concentrations of ATRA on BMP9-induced matrix mineralization in MEFs after 21 days. l ALP staining showed the effect of different concentrations of ATRA on OIM-induced ALP activities in MEFs after 9 and 11 days. m Quantification of ALP staining demonstrated the effect of different concentrations of ATRA on OIM-induced ALP activities in MEFs after 9 and 11 days, *p < 0.05 vs OIM + DMSO, **p < 0.01 vs OIM + DMSO. GFP, green fluorescent protein; DMSO, dimethyl sulfoxide; DMEM, Dulbecco’s modified Eagle’s medium; OIM, osteogenic inductive medium

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