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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: The role of Serpina3n in the reversal effect of ATRA on dexamethasone-inhibited osteogenic differentiation in mesenchymal stem cells

Fig. 7

Effects of Serpina3n overexpression and knockdown on the reversal process of ATRA in DEX-inhibited osteogenic differentiation. a MEFs infected with adenovirus-mediated Ad-RFP, Ad-siSerpina3n, and Ad-Serpina3n after 24 h. b Western blot showed the protein expression of Serpina3n in MEFs 24 h after Ad-RFP, Ad-siSerpina3n and Ad-Serpina3n infection. c ALP staining demonstrated the effect of overexpression of Serpina3n on ATRA-induced ALP activities in MEFs after 5 and 7 days. d Quantification of ALP staining showed the effect of overexpression of Serpina3n on ATRA-induced ALP activities, *p < 0.05, **p < 0.01. e ALP staining showed the effect of knockdown of Serpina3n on DEX-inhibited ALP activities induced by BMP9. f Quantification of ALP staining showed the effect of knockdown of Serpina3n on DEX-inhibited ALP activities, *p < 0.05, **p < 0.01. g Western blot results showed the effect of knockdown of Serpina3n on DEX-inhibited osteogenic marker ALP and RUNX2 induced by BMP9 in MEFs. h Quantification of western blot demonstrated the effect of knockdown of Serpina3n on DEX-inhibited osteogenic markers in MEFs, *p < 0.05, **p < 0.01. i ALP staining showed the effect of Serpina3n overexpression and knockdown on ATRA-reversed ALP activities in MEFs. j Quantification of ALP staining showed the effect of Serpina3n on ATRA-reversed ALP activities, *p < 0.05, **p < 0.01. GFP, green fluorescent protein; RFP, red fluorescent protein; DMSO, dimethyl sulfoxide; DEX, dexamethasone

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