Fig. 8

Effects of RARβ on the expression of Serpina3n in the reversal process of ATRA to DEX. a RTqPCR results showed the effect of Le135 and ATRA on the expression of Serpina3n, *p < 0.05 vs Le135, **p < 0.01 vs Le135. b Western blot results demonstrated the effect of RARβ inhibitor and ATRA on the protein expression of Serpina3n. c Quantification of western blot results showed the effect of Le135 and ATRA on the expression of Serpina3n, *p < 0.05, **p < 0.01. d Possible reversal mechanism of ATRA on DEX-induced osteogenic differentiation. DEX can be transferred into the nucleus to promote the transcription of Serpina3n; once ATRA is transferred into the nucleus by CRABPII, it will bind to RARβ to further modulate the transcription of Serpina3n. CYP26 is for degradation of ATRA transferred by CRABPI. DMSO, dimethyl sulfoxide; DEX, dexamethasone; Le135, specific inhibitor of RARβ; GR, glucocorticoid receptor; CRABPI, cellular retinoic acid binding protein I; CRABPII, cellular retinoic acid binding protein II; CYP26, cytochrome P450 26