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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: DPSCs treated by TGF-β1 regulate angiogenic sprouting of three-dimensionally co-cultured HUVECs and DPSCs through VEGF-Ang-Tie2 signaling

Fig. 8

The expression of p-Tie2 and p-VEGFR2 in HUVECs. a–c Western blot analysis of the expression of p-Tie2 and Tie2 in HUVEC/T-DPSC co-culture with or without high concentration Ang2 (1000 ng/mL) for 24 h. d, e HUVECs were pretreated with the Tie2 inhibitor (5 μM) for 12h, then treated with T-DPSC-CM or Ang1 for 30 min. Western blot analysis of the expression of p-Tie2 in HUVECs. f, g HUVECs were pretreated with the VEGFR2 inhibitor (5 μM) for 12h, then treated with VEGF for 2, 5, and 10 min. Western blot analysis of the expression of p-VEGFR2 in HUVECs. Data are mean ± standard error for n = 3 replicates, *p <0.05, **p <0.01, ***p <0.001, ****p <0.0001

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Stem Cell Research & Therapy

ISSN: 1757-6512