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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Conversion of stem cells from apical papilla into endothelial cells by small molecules and growth factors

Fig. 2

Characterization of endothelial identity after SM-based lineage conversion of SCAP. a mRNA expression of endothelial-specific markers in non-treated SCAP, SCAP in EGM2, SCAP-ECs and HUVECs. b, c Protein expression levels of endothelial-specific markers in SCAP, SCAP in EGM2, SCAP-ECs and HUVECs. d The SCAP-ECs were immune-positive for CD31, VEGFR2 and vWF, with HUVECs as control. Scale bar = 100 μm. e Flow cytometry analysis of SCAP-ECs showed relatively higher expression levels of VEGFR2, TIE2 and CD31, with HUVECs as control. f EC-specific transcriptome profiles of SCAP, SCAP-ECs and HUVECs. g GO analysis of upregulated genes of SCAP-ECs versus SCAP. All of the results represent the mean ± SEM of 3 independent experiments (n = 3). *P < .05, **P < .01

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