Fig. 3

The in vitro functional assessment of SCAP-ECs. a, b The tube-like structure formation on Matrigel assay. Scale bar = 500 μm. a At 12 h, SCAP-ECs showed a significantly higher number of nodes, junctions, meshes and total tube length than the EGM-2 group and SCAP. b At 24 h, only SCAP-ECs and HUVECs maintained an intact tubular network. c Immunofluorescence for Dil ac-LDL. Ac-LDL uptake was detected in SCAP-ECs. Scale bar = 50 μm. d Pericytes (green) were localized along tube-like structures formed by both SCAP-ECs (red) and HUVECs (red). Scale bar = 200 μm. e The ability of NO generation in SCAP-ECs, SCAP in EGM-2 group and non-treated SCAP and HUVECs. All of the results represent the mean ± standard deviation of 3 independent experiments (n = 3). ^*P < .05, ^**P < .01