Fig. 2

Flow cytometric histograms of adipose-derived stem cells (ADSCs) within the stromal vascular fraction (SVF) of a representative patient. Each histogram contains an isotype-matched negative Ab control (gray line) and Abs against the following Ags (black line). Representative fluorescent dot plot displaying cells within the freshly isolated lipoaspirate (a). Dot plot displaying mesenchymal stem cell (MSC) identified using antibodies directed against CD45, CD31, and CD34 (b) and negative control (c). Two-dimensional histograms displaying staining for CD45-FITC, the pan-hematopoietic marker (d); CD31-PE, found on endothelial cells, platelets, and leukocytes (e); and CD34-PC, a marker for pluripotent stem cells (f). Histogram of identical ADSC stained with anti-CD44 Abs conjugated to PE or FITC