Fig. 3
From: A novel strategy for isolation of mice bone marrow endothelial cells (BMECs)
Characterization of primary bone marrow endothelial cells by flow cytometry: a the primary BMECs were negative for CD 45 (98.2%). b CD 31 (80.6%), c CD106 (7576%), d CD144 (64.4%), and e endothelial selective adhesion molecules (ESAM) (50.6%). The red tented histograms represent the unstained cells as the isotype control, and the colored histograms indicate the antigen staining of the endothelial cell’s surface markers. The bar chart indicates the mean ± SD using Student’s t test of two experimental repeats compared to the isotype control [N =2 with 6 mice/group (12 femora and 12 tibias)] and p value ≤ 0.05 and considered statistically significant. For CD 45, there is no statistical significance between the negative isotype control (unstained cells) and the stained cells, which verify the purity of the cells isolated are the characteristics of bone marrow endothelial cells, as endothelial cells express CD45-negative molecule. **p<0.01, ***p<0.001, and NS not significant