Table 1 Published methods for isolation of endothelial cells from the bone marrow
From: A novel strategy for isolation of mice bone marrow endothelial cells (BMECs)
References | Type of species | Method of isolation | Purification method |
|---|---|---|---|
Peter A G McCourt [13] | Mouse | Mechanically digestion of bone marrow cells | Magnetic beads labeling Advanced glycation end-products (AGE)/fluorescence-activated cell sorting (FACS) |
S Irie [14]. | Rat | Collagenase digestion of bone marrow cells | Discontinuous gradient centrifugation on Percoll (densities 1.04 and 1.06) |
Nakatsuka, Ryusuke [15] | Murine | Enzymatic digestion of murine bone | Fluorescence-activated cell sorting (FACS) |
Yuxin Feng [16] | Mouse | Not available | Fluorescence-activated cell sorting (FACS) |
Van Beijnum, Judy R. Rousch, Mat [17] | Human/other species. | Mechanically minced and enzymatic digestion with collagenase and dispase | Single magnetic beads cell suspension/ high-speed cell sorting |