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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Human placental mesenchymal stem cells ameliorate liver fibrosis in mice by upregulation of Caveolin1 in hepatic stellate cells

Fig. 3

The activation of HSCs was inhibited by hPMSC in vitro. a Representative western blot of α-SMA and GAPDH of activated HSCs, in the presence of concentrated MSC medium or concentrated hPMSC culture supernatant (hPMSC secretomes). b Typical cell morphology (upper) and α-SMA immunofluorescence staining (lower) of HSCs. c Expression of fibrosis-related genes in activated HSCs was determined using qRT-PCR. Relative mRNA expression was normalized to β-actin and compared with the TGF-β1 group. Cells from blank group were unactivated HSCs, cells from TGF-β1 group were activated HSCs that induced by TGF-β1, and cells from medium group or hPMSC group were activated HSCs treated with either concentrated MSC medium or hPMSC secretomes. Scale bar, 50 μm. ****p < 0.0001, ***p < 0.001, **p < 0.01; *p < 0.05; ns, no significance. HSCs, hepatic stellate cells

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Stem Cell Research & Therapy

ISSN: 1757-6512