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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Human placental mesenchymal stem cells ameliorate liver fibrosis in mice by upregulation of Caveolin1 in hepatic stellate cells

Fig. 7

hPMSCs inhibit TGF-β/Smad signaling pathway by restoring the function of Cav1 in HSCs. a HSCs were transfected with Cav1 siRNA 1-2 and Cav1 siRNA-NC, respectively. Transfected HSCs were treated with TGF-β1 and then cultured with or without hPMSC secretomes (40%). Expression of Cav1 and fibrosis-related genes (Acta2, Col1a1, Desmin) at different groups was determined using qRT-PCR. Relative mRNA expression was normalized to β-actin and compared with the TGF-β1 group (column 2). b Representative western blot of CAV1, α-SMA, and Smad2 from HSCs at different groups. Cells from TGF-β1 group were activated HSCs, without extra treatment. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05; ns, no significance

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