Table 1 Primer sequences for qPCR
From: Vitamin D3 suppresses intestinal epithelial stemness via ER stress induction in intestinal organoids
Primers | Forward sequences (5′ to 3′) | Reverse sequences (5′ to 3′) |
|---|---|---|
GAPDH | TTG ATG GCA ACA ATC TCC AC | CGT CCC GTA GAC AAA ATG GT |
Ki67 | CCA GCT GCC TGT AGT GTC AA | TCT TGA GGC TCG CCT TGA TG |
LGR5 | ACC CGC CAG TCT CCT ACA TC | GCA TCT AGG CGC AGG GAT TG |
LYZ | GAG ACC GAA GCA CCG ACT ATG | CGG TTT TGA CAT TGT GTT CGC |
MUC2 | ATG CCC ACC TCC TCA AAG AC | GTA GTT TCC GTT GGA ACA GTG AA |
CHGA | AAG GTG ATG AAG TGC GTC CT | GGT GTC GCA GGA TAG AGA GG |
VIL | GAC GTT TTC ACT GCC AAT ACC A | CCC AAG GCC CTA GTG AAG TCT T |
BRG1 | CAG TGG CTC AAG GCT ATC G | TGT CTC GCT TAC GCT TAC G |
NOTCH1 | AGT GTG ACC CAG ACC TTG TGA | AGT GGC TGG AAA GGG ACT TG |
CHOP | CTG CCT TTC ACC TTG GAG AC | CGT TTC CTG GGG ATG AGA TA |
ATF6 | CCA ACA GAA AGC CCG CAT T | TGG ACA GCC ATC AGC TGA GA |
tXBP1 | AAG AAC ACG CTT GGG AAT GG | ACT CCC CTT GGC CTC CAC |
sXBP1 | GAG TCC GCA GCA GGT G | GTG TCA GAG TCC ATG GGA |