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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Induced pluripotent stem cell-derived mesenchymal stem cells deliver exogenous miR-105-5p via small extracellular vesicles to rejuvenate senescent nucleus pulposus cells and attenuate intervertebral disc degeneration

Fig. 3

iMSC-sEVs can ameliorate the senescent phenotype of NPCs and age-related dysfunction in vitro. a IF analysis of DiO-labelled sEV internalization by NPCs. Scale bar, 50 um. b Representative micrographs of NPCs stained with SA-훽-gal in different treatment groups. SA-훽-gal-positive cells are shown in blue. Scale bar, 150 um. c Percentage of SA-훽-gal-positive cells. n = 3 per group. d CCK-8 analysis of NPC proliferation in different treatment groups. n = 5 per group. e The expression of P16 was assessed by Western blotting. f Densitometric quantification of the relative band intensity in e. n = 3 per group. g The expressions of collagen II, aggrecan, MMP-3, and ADAMTS-4 were assessed by Western blotting. h Densitometric quantification of the relative band intensity in g. n = 3 per group. *P<0.05, **P< 0.01, ***P< 0.001

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