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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Induced pluripotent stem cell-derived mesenchymal stem cells deliver exogenous miR-105-5p via small extracellular vesicles to rejuvenate senescent nucleus pulposus cells and attenuate intervertebral disc degeneration

Fig. 5

iMSC-sEVs downregulate PDE4D expression and activate the Sirt6 signalling pathway by delivering miR-105-5p into senescent NPCs. a Heatmap of the top 10 highest differential expressing miRNAs between iMSC-sEVs and FB-sEVs. b qRT-PCR validated the levels of the 10 upregulated miRNAs. c Schematic representation of the predicted PDE4D 3′-UTR indicating the has-miR-105-5p binding sites and the designed mutated versions of PDE4D 3′-UTR. d Luciferase activities in cells transfected with miR-105-5p mimics or negative control. e The expression of PDE4D was assessed by Western blotting. f Densitometric quantification of the relative band intensity in e. n = 3 per group. *P<0.05, **P< 0.01, #P< 0.001; n.s, P> 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512