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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Induced pluripotent stem cell-derived mesenchymal stem cells deliver exogenous miR-105-5p via small extracellular vesicles to rejuvenate senescent nucleus pulposus cells and attenuate intervertebral disc degeneration

Fig. 7

PDE4D overexpression in NPCs blocks the therapeutic effect of iMSC-sEVs on senescent NPCs. a IF analysis of NPCs transfected with GFP-labelled adenovirus under different MOI values. Scale bar, 150 um. b The expressions of PDE4D and Sirt6 were assessed by Western blotting. c Densitometric quantification of the relative band intensity in b. n = 3 per group. d Representative micrographs of NPCs stained with SA-β-gal in different treatment groups. SA-β-gal-positive cells are shown in blue. Scale bar, 150 um. e Percentage of SA-β-gal-positive cells. n = 3 per group. f CCK-8 analysis of NPC proliferation in different treatment groups. n = 5 per group. g The expression of P16 was assessed by Western blotting. h Densitometric quantification of the relative band intensity in g. n = 3 per group. i The expressions of collagen II, aggrecan, MMP-3, and ADAMTS-4 were assessed by Western blotting. j Densitometric quantification of the relative band intensity in i. n = 3 per group. *P<0.05, **P< 0.01, ***P<0.001

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