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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Mesenchymal stem cell-derived microvesicles improve intestinal barrier function by restoring mitochondrial dynamic balance in sepsis rats

Fig. 5

MMVs delivered mfn2 to IEC-6 to improve the mitochondrial function. a The relative expression of mfn1, mfn2, and OPA1 in MSCs, MMVs, and CMVs. β-actin was used as the internal reference (n=5). b The relative expression of mfn2 in IEC-6 treated by MMVs. β-actin was used as the internal reference (n=5). c The relative expression of mfn2 in IEC-6 treated by modified MMVs. β-actin was used as the internal reference (n=5). d Representative microphotographs of mitochondria treated by modified MMVs and the average mitochondria length in 30 cells. The scale bar represents 10μm. e Representative microphotographs of ΔΨm in IEC-6 treated by modified MMVs. The scale bar represents 10μm. f Representative microphotographs of ROS in IEC-6 treated by modified MMVs. The scale bar represents 50μm. g ATP concentration in IEC-6 treated by modified MMVs (n=6). h OCR in IEC-6 treated by modified MMVs (n=6). Ctl, normal control group; LPS, LPS-stimulated group; CMV, CMV-treated group; MMV, MMV-treated group; MMVmfn2-up, mfn2 upregulation in MMVs; MMVmfn2-down, mfn2 downregulation in MMVs; MMVvehicle, mfn2-mocktransfection in MMVs. **P < 0.01, *P < 0.05

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