Fig. 6
MMVs delivered mfn2 to IEC-6 to improve the proliferation and barrier function. a Effect of modified MMVs on TEER of IEC-6 (n=5). b Effect of modified MMVs on BSA leakage of IEC-6 (n=6). c Effect of modified MMVs on CCK8 proliferation assay of IEC-6 (n=6). d The relative expression of PCNA in IEC-6 treated by modified MMVs. β-actin was used as the internal reference (n=5). e Representative microphotographs of scratch migration assay and the scratched area of IEC-6 treated by modified MMVs. The scale bar represents 100μm. f Representative IF microphotographs of ZO-1 in IEC-6 treated by modified MMVs. The scale bar represents 20μm. Ctl, normal control group; LPS, LPS-stimulated group; CMV, CMV-treated group; MMV, MMV-treated group; MMVmfn2-up, mfn2 upregulation in MMVs; MMVmfn2-down, mfn2 downregulation in MMVs; MMVvehicle, mfn2-mocktransfection in MMVs. **P < 0.01, *P < 0.05