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Fig. 9 | Stem Cell Research & Therapy

Fig. 9

From: Mesenchymal stem cell-derived microvesicles improve intestinal barrier function by restoring mitochondrial dynamic balance in sepsis rats

Fig. 9

MMVs delivered functional mitochondria to IEC-6. a Flow cytometry analysis of mitochondria-positive MMVs detected by mitotracker-Red. b Representative TEM microphotographs of mitochondria-carried MMVs. The scale bar represents 500nm. c Flow cytometry analysis of ΔΨm in MMVs. d OCR analysis in MMVs (n=5). e Representative confocal microphotographs of the fusion of MMV-delivered mitochondria (Green pseudocolor) and the mitochondria in IEC-6 (Red pseudocolor). The scale bar represents 10μm. f The relative fluorescence intensity of MMVs and the co-location rate of two mitochondria in IEC-6 (n=5). g Flow cytometry analysis of mitochondria-positive MMVs in PGC-1α upregulated MMVs (n=5). **P < 0.01

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