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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Development, characterization, and hematopoietic differentiation of Griscelli syndrome type 2 induced pluripotent stem cells

Fig. 3

Immunophenotype of BM-MNC- and BM-MSC-derived iPSC clones. a Top lane: representative histograms of a GS-2 iPSC clone (YF #D2B2) derived from BM-MNCs. Unstained controls are given as black lines and stained iPSC as red lines. Lower lane: representative histograms and dot plots from a BM-MSC-derived GS-2 iPSC clone (YF #A2C3). b Healthy donor iPSc (top) and GS-2 iPSC (bottom) immunofluorescence images, representative samples. Both healthy donor and GS-2 iPSCs show bright expression of pluripotency markers. The nuclei were counterstained with DAPI. c Relative gene expression levels of SOX2, NANOG, and OCT4 normalized to B2M expression. Healthy (GP) and GS-2 (IK, YF, YKÇ) iPSC clones showed increased levels of SOX2, NANOG, OCT4 gene expression in comparison with healthy donor (LA) and GS-2 (YKÇ) BM-MSCs (negative controls)

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