Fig. 3

Treatment with iMSC-CdM inhibited inflammation and enhanced angiogenesis. a Trichrome staining revealed that the wounds treated with uMSC-CdM and iMSC-CdM had similar granulation tissue at the peri-wound area (arrows indicate keratinocytes; stars indicate granulation tissue). Scale bar = 100 μm. b Inflammatory cytokine expression was determined by a bead-based immunoassay. c Representative fluorescence images and quantification of CD31 positive blood vessels. Scale bar = 100 μm. d Expression of pro-angiogenesis factors at the peri-wound area determined by quantitative polymerase chain reaction. e EGF and VEGFA protein expression determined by Elisa assay. ns, non-significance, *P < .05, **P < .01, ***P < .001