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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: The stromal vascular fraction mitigates radiation-induced gastrointestinal syndrome in mice

Fig. 6

SVF promoting anti-inflammatory monocyte/macrophage subsets in the spleen. a Phalloidine staining (green) of splenic monocytes from the control and at day 7 post-irradiation with or without SVF treatment. Dapi stained nuclei. Scale bar 20 μm. The bar graphs are represented by the mean ± SEM of monocytes phalloidine+ and the monocyte diameters. b Representative FACS plots and frequencies of CD11b+Ly6ChighCCR2high and CD11b+Ly6ClowCx3cr1high among live cells from the control and at day 7 post-irradiation with or without SVF treatment. c Cx3cr1staining (red) of splenic monocytes. The bar graphs are represented by the mean ± SEM of Cx3CR1+ monocytes. Dapi stained nuclei. Scale bar 20 μm. d Gene expression from splenic monocytes differentiated in different culture conditions from the control and at day 7 post-irradiation with or without SVF treatment. Macrophages were generated from splenic monocytes in the presence of M-CSF. Real-time PCR in an unstimulated condition, a M1 condition after stimulation with LPS and IFN-γ and a M2a condition after stimulation with IL-4. The data are expressed relative to control and normalised to GAPDH. All results are expressed as means ±SEM (n = 8). p values were calculated by ANOVA with Bonferroni correction, *p < 0.05; **p < 0.01; ***p < 0.001 compared with the control group; #p < 0.05; ##p < 0.01; ###p < 0.001 compared with the irradiated group

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