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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Hypoxic mesenchymal stem cells ameliorate acute kidney ischemia-reperfusion injury via enhancing renal tubular autophagy

Fig. 2

Effect of hypoxic rat mesenchymal stem cells (HMSCs) on oxidative stress induced by renal ischemia-reperfusion (I/R) injury. a In vivo detection of reactive oxygen species (ROS) in the rat kidneys subjected to sham-operation (Sham), ischemia-reperfusion surgery followed by intra-renal arterial injection (IA) of either phosphate-buffered saline (I/R+PBS), normoxia-cultured mesenchymal stem cells (I/R+MSC, 5 × 105 cells per rat), or HMSCs (I/R+HMSC, 5 × 105 cells per rat) was measured immediately after reperfusion. The amount of ROS was expressed as chemiluminescence counts/10 s. Please see the “Material and methods” for details. b Quantification of in vivo detection of the reactive oxygen species in the rat kidneys of Sham, I/R+PBS, I/R+MSC, and I/R+HMSC groups. **p < 0.01 by one-way ANOVA with Tukey’s post hoc comparison. ns, nonsignificant; n = 3 per group. c Representative periodic acid-Schiff stained images of kidney sections from rats subjected to sham operation (Sham group), renal ischemia for 45 min followed by 48 h of reperfusion and treated with either PBS, MSCs, or HMSCs at the onset of reperfusion. The I/R injury induced diffuse denudation of the renal tubular cells with loss of brush border (arrows) and flattening of tubular cells with intratubular cast formation (yellow arrowheads) in the I/R+PBS group. The extent of acute tubular injury (arrows) were decreased in the I/R+IA-MSC and I/R+IA-HMSC groups. Scale = 100 μm. d Quantification of the renal tubular injury. **p < 0.01, ***p < 0.001 by one-way ANOVA with Tukey’s post hoc comparison, n = 5 per group. e, f Serum blood urea nitrogen and creatinine levels in the Sham, I/R+PBS, I/R+IA-MSC, and I/R+IA-HMSC groups at 48 h after reperfusion. **p < 0.01, ***p < 0.001 by one-way ANOVA with Tukey’s post hoc comparison, n = 5 per group

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