Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: WNT5A from the fetal liver vascular niche supports human fetal liver hematopoiesis

Fig. 1

The human fetal liver contains immature CD45CD43+ hematopoietic cells that can mature to multilineage CD45+CD34+ HSPC. A CD45CD144CD43+CD235a hematopoietic progenitor cells were detected at significantly higher frequency in the fetal liver than other fetal tissues. (i) CD45CD144CD43+CD235a cells were detected during in vitro human embryonic stem cell (hESC) differentiation (see also supplemental figure 1). (ii, iii) CD45CD144CD43+CD235a cells within the human fetal heart (ii) and liver (iii). (iv) Quantification of the percentage of CD45CD144CD43+CD235a HPCs among four fetal tissues (n = 3 donors, mean ± SEM, ***p < 0.001). B Schematic diagram of CD45 CD144CD43+CD235a cells sorted by FACS and cultured on subconfluent E4ORF1-transduced primary human umbilical vein endothelial cells (E4ECs) in serum-free media supplemented with hematopoietic cytokines for 10 days. C, D Following E4EC co-culture, CD45CD144CD43+CD235a cells generate (C, i) CD45+CD34+ cells as well as (D, i) colony-forming progenitors including CFU-GM, CFU-GEMM, and BFU-E (scale bar, 2000 μm). Quantification of CD45+CD34+cells (C, ii) and CFC assay (D, ii) (n = 3 donors mean ± SEM). E CD45CD43+ HPCs from human fetal liver can differentiate into T lymphocytes. (i) Following primary culture of HPC on E4EC stroma, hematopoietic progeny was co-cultured with OP9-Dll4 for 14–21 days, and the induced non-adherent cells were characterized with early T cell markers CD5 and CD7 via flow cytometry. (ii) Quantification of CD5+CD7+ cells (n = 3 donors mean ± SEM)

Back to article page