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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Human amnion-derived mesenchymal stem cells improved the reproductive function of age-related diminished ovarian reserve in mice through Ampk/FoxO3a signaling pathway

Fig. 3

hAMSCs were homing in ovary and improved reproductive function after transplantation. a–d hAMSC homing and differentiation were traced by immunofluorescence experiments. Human-specific antibody of STEM121 (green signal) and mesenchymal stem cell-specific surface marker of CD73 (red signal) co-localization in the ovary. The sections were from middle dose of hAMSCs transplanted in ovary. Blue signal: 4,6-diamidino-2-phenyiindole (DAPI) staining. Scale bar 100 μm. e Serum levels of sex hormone were analyzed by ELISA kit. The AMH level was significantly increased and the FSH level was significantly decreased in the MD group. f Histological analysis of hematoxylin and eosin (HE) staining was performed to observe ovary. There were more follicles in the MD and HD groups. Scale bar, 500 μm. g Follicle counting showed the number of primordial and primary follicles in the HD group, and secondary and antral follicles in the MD and HD groups were significantly increased. h Retrieval oocyte counting indicated there was no significant difference in the numbers of oocyte between different groups. All above assays were performed at day 7 post-transplantation of hAMSCs. AMH: anti-Mullerian hormone; E2: estradiol; FSH: follicle stimulating hormone. NS: normal saline group; HSA: 1% human serum albumin group; LD: low-dose group; MD: middle-dose group; HD: high-dose group. n = 8. error bars indicate SEM. Different lowercase letters represent significantly statistical differences (P < 0.05)

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