Skip to main content
Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Senescence of donor cells impairs fat graft regeneration by suppressing adipogenesis and increasing expression of senescence-associated secretory phenotype factors

Fig. 6

Transcriptomic analysis of fat grafts from the O-O and Y-O groups. a Volcano plot of DEGs between fat grafts at week 1 in the O-O and Y-O groups. –log10 padj and log2 (fold change) values are shown. b Enriched GO terms including “adipogenesis” and “tissue inflammation” associated with the DEGs in fat grafts from old donors compared with young donors. To capture the overall tendency toward upregulation or downregulation of each term, a z-score was calculated (\( Z=\frac{\left(\mathrm{up}-\mathrm{regulated}-\mathrm{down}-\mathrm{regulated}\right)}{\sqrt{\mathrm{total}}\mathrm{DEGs}} \)) and is displayed coded by color. Relative gene expression levels of the GO terms c “white fat cell differentiation” and d “regulation of inflammatory response” are presented in heat maps. Expression levels of e Pparg, f Tnf, g Il1b, and h Il6 were validated using RT-PCR in fat grafts at weeks 1, 4, and 8 from old and young donors. Data are presented as the mean ± SD (n = 6 per group)

Back to article page