Fig. 2
Characterization, differentiation, and migration of bone marrow stem cells (BMSCs). A A representative image showing fibroblastic morphology of BMSCs (× 200). B Flow cytometry curve showing a control, left side: gating of unstained cells at FITC, right side: gating of unstained cells at PE. C Flow cytometry curve showing the expression of CD105 in BMSCs. D Flow cytometry curve showing the expression of CD73 in BMSCs. E Flow cytometry curve showing the expression of CD34 in BMSCs. F Flow cytometry curve showing the expression of CD14 expression in BMSCs. G Flow cytometry curve showing the expression of CD63 in exosome produced by BMSCs. H Control BMSCs cultured in LDMEM supplemented with 10% bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin, left side: stained with Oil Red and right side: stained with Alizarin Red (× 200). I BMSCs stained with Oil Red showing adipogenic differentiation of BMSCs (× 200). J BMSCs stained with Alizarin Red showing osteogenic differentiation of BMSCs (× 200). K Transmission electron microscope image of exosome produced by BMSCs showing vesicular structure (arrow). L Localization of PKH26-labeled BMSCs in the hippocampal regions of the brain tissues of rats (× 200) (n = 4)