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Table 1 Sequences of primers sets used for gene expression analysis

From: Bone marrow mesenchymal stem cells and their derived exosomes resolve doxorubicin-induced chemobrain: critical role of their miRNA cargo

Gene symbol Primer sequence GenBank accession number
Olig2 F:
Olig2 R:
5′- TTCAGACCACATGAGCAAGC-3′
5′- TAAAAACAGCGTCCCCAGTC-3′
NM_001100557.1
Opalin F:
Opalin R:
5'-AAGTGTATCCCAGCTTGCCT-3'
5'-CTTCCCGATGTCTCTCCCTC-3'
NM_001017386.1
FGF-2 F:
FGF-2 R:
5’-CCCACACGTCAAACTACAGC-3'
5'-CTGCCCAGTTCGTTTCAGTG-3'
NM_019305.2
Syp F:
Syp R:
5′-ACAGGAAGGGAACCAGACCT-3′
5′-CAAGCCTCCTCCACTCAGTC-3′
NM_012664.3
Shh F:
Shh R:
5′- CAATTACAACCCCGACATCA-3′
5′-AGTCACTCGAAGCTTCACTCC-3′
NM_017221.1
Ptch-1 F:
Ptch-1 R:
5′-ACGCTCCTTTCCTCTTGAGAC-3′
5′-TGAACTGGGCAGCTATGAAGT C-3′
NM_053566.1
Gli-1 F:
Gli-1 R:
5′-CAGGGAAGAGAGCAGACTGAC -3′
5′-CAGGAGGATTGTGCTCCA -3′
NM_001191910.1
WNT2 F:
WNT2 R:
5′- AGGCAGCGTTTGTCTATGCT-3′
5′- GTCACTACAGCCACCCCAGT-3′
XM_575397.7
WNT3a F:
WNT3a R:
5′- CCTGGTTGGGGTCAGTAAGA-3′
5′- GGTAGAGAGTGCAGGCAAGG-3′
NM_001107005.2
FZD1 F:
FZD1 R:
5′- GCGACGTACTGAGCGGAGTG-3′
5′- TGATGGTGCGGATGCGGAAG-3′
NM_021266.3
Neurod1 F:
Neurod1 R:
5′- ACAGCTCCCATGTCTTCCAC-3′
5′- AAGATTGATCCGTGGCTTTG-3′
NM_019218.2
Prox1 F:
Prox1 R:
5′- GAAGGGCTATCACCCAATCA-3′
5′- TGGACAGTTCCTCTGTGCTG-3′
NM_001107201.1
NeuN F:
NeuN R:
5'-TTGCTTCCAGGGTCGTGTAT-3'
5'-GGGCCGATGGTATGATGGTA-3'
NM_001134498.2
DCX F:
DCX R:
5′- CCTTCAATGTCATGGCACAC-3′
5′- CTCTGGCTTGGCTCACTACC-3′
NM_053379.3
Sox2 F:
Sox2 R:
5'-ATTACCCGCAGCAAAATGAC-3'
5'-ATCGCCCGGAGTCTAGTTCT-3'
NM_001109181.1
Cx3cr1 F:
Cx3cr1 R:
5'-CCAACTCCATGAACAACCGG-3'
5'-GATGTTGACCTCCGAGTTGC-3'
NM_133534.1
Bax F:
Bax R:
5′-GATCAGCTCGGGCACTTTA-3′
5′-TGTTTGCTGATGGCAA CTTC-3′
NM_017059.2
Bcl2 F:
Bcl2 R:
5′-AGGAT TGTGG CCTTC TTTGA GT-3′
5′-GCCG GTTC AGG TACT CAGT CAT-3′
NM_016993.1
β-actin F:
β-actin R:
5′- TGTCACCAACTGGGACGATA-3′
5′- GGGGTGTTGAAGGTCTCAAA-3′
NM_031144.3
  1. Olig2, oligodendrocyte transcription factor-2; Opalin, oligodendrocytic paranodal loop protein; FGF-2, fibroblast growth factor-2; Syp, Synaptophysin; Shh, Sonic hedgehog; Ptch-1, patched-1; Gli, glioma-associated oncogene family zinc finger; Wnt, wingless-type MMTV integration site family; FZD1, frizzled1; Neurod1, neurogenic differentiation 1; Prox1, prospero-related homeobox; NeuN, hexaribonucleotide binding protein-3; DCX, doublecortin; SOX2, sex-determining region Y-box 2; β-actin, beta-actin; Cx3cr1, CX3C chemokine receptor 1; Bcl2, B cell lymphoma 2; BAX, BCL2-associated X