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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Extracellular vesicles containing miR-146a-5p secreted by bone marrow mesenchymal cells activate hepatocytic progenitors in regenerating rat livers

Fig. 1

Characterization of BM-MCs and EVs. a Phase-contrast image of BM-MCs at day seven after plating. b Microscopic images of BM-MCs induced to undergo adipogenesis (Oil-Red; oil red-O staining), osteogenesis (Alp; alkaline phosphatase activity staining), and chondrogenesis (Alcian blue staining). Scale bar, 100 μm. c Flow-cytometry analysis of cell surface markers (CD90, CD73, CD29, CD44, CD34, CD11b, CD45) present on BM-MCs. d EVs derived from CM of cultured BM-MCs after 48 h were analyzed using the NanoSight particle tracking system. Overall size distribution (histograms) and mode (nm). e Sample video frame. f Western blots show the existence of CD63, HSP70, and actin by EVs, CM (CM-EVs), and cell lysis (BM-MCs). Spleen (SP) is used as positive control

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Stem Cell Research & Therapy

ISSN: 1757-6512