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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Extracellular vesicles containing miR-146a-5p secreted by bone marrow mesenchymal cells activate hepatocytic progenitors in regenerating rat livers

Fig. 4

Effects of soluble factors derived from BM-MCs on the colony formation of small hepatocytes. a Photos show the immunohistochemistry for BrdU of typical SH colonies at 7 days after plating. SHs were indirectly cocultured with BM-MCs by using a cell strainer. Brown nuclei were BrdU-positive, most of which are mononucleate. Scale bars, 100 μm. b The number of cells per colony was measured. c The percentage of BrdU+ cells per colony is calculated. Bars show SE. Asterisks indicate statistically significant differences; p < 0.05. Panel d shows the photos of typical SH colonies treated with EVs and CM-EVs derived from BM-MCs at 7 days after the treatment. Immunocytochemistry for BrdU and hematoxylin-staining were performed. Scale bars, 100 μm. The number of SH colonies (e), the number of cells per colony (f), and the percentage of BrdU+ cells per colony (g) are measured. Bars show SEs. Asterisks indicate statistically significant differences; p < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512