Fig. 5

Administration of EVs secreted by cultured BM-MCs to Ret/PH model rat livers. EVs and CM-EVs derived from the conditioned medium of cultured BM-MCs for 48 h were administered via spleen, and the livers were examined at 14 days post-administration. a Liver and body weights of rats treated with EVs or CM-EVs were measured 14 days after the treatment. Bars show SE. Asterisks indicate statistically significant differences; p < 0.05. b Photos of SHPC clusters in the livers of control (left), EVs (center), and CM-EVs (right). The samples were stained with H-E. Scale bar, 200 μm. c The number of SHPC clusters per liver area and d the number of cells per cluster is shown. Bars show SEs and asterisks indicate statistically significant differences, p < 0.05