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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Extracellular vesicles containing miR-146a-5p secreted by bone marrow mesenchymal cells activate hepatocytic progenitors in regenerating rat livers

Fig. 6

Effects of miRNAs abundantly contained in EVs secreted by BM-MCs on the proliferation of SHs. a Six miRNAs up-regulated 5 times higher than those of hepatic Thy1+ cells are shown as a heatmap. b Amounts of miR-144-3p, miR-146a-5p, miR-146b-5p, miR-221-3p, miR-222-3p, and miR-375-5p in the EVs secreted by SHs, hepatic Thy1+ cells, and BM-MCs were confirmed by using qRT-PCR. Four of six miRNAs were included more abundantly in EVs secreted by BM-MCs than in other cells. Bars show SEs and asterisks indicate statistically significant differences, p < 0.05. c These photos show the SH colonies, which mimics those 4 miRNAs, miR-146a-5p, miR-146b-5p, miR-221-3p, and miR-222-3p, were transfected by adding to the culture medium for 2 days. The cells were then cultured until day seven and BrdU was added in culture medium 24 h before fixation. Immunocytochemistry for BrdU was conducted. Scale bars, 100 μm. The total number of SH colonies per well (d) and the number of cells per colony (e), and the percentage of BrdU+ cells per colony (f) were measured. Asterisk indicates statistically significant differences compared with control, p < 0.05

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