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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Extracellular vesicles containing miR-146a-5p secreted by bone marrow mesenchymal cells activate hepatocytic progenitors in regenerating rat livers

Fig. 7

Effects of cytokines included in EVs derived from BM-MCs. a The amounts of cytokines included in EVs secreted from hepatic Thy1+ cells, BM-MCs and MHs were measured using a custom-designed Quantibody rat-specific protein array (QAR-CAA-67). SCF, IL-6, and IL-13 were included in EVs secreted from BM-MCs much more than ones from Thy1+ cells, and IL-6 and IL-13 were included in EVs secreted from BM-MCs much more than ones from MHs. b SCF, IL-6, and IL-13 were added to the culture medium 3 h after plating and refreshed every other day. Panel b shows the photos of typical SH colonies treated with each cytokine. BrdU was added to the culture medium 24 h before fixation, and immunocytochemistry for BrdU was performed at 7 days after plating. Scale bars, 100 μm. The total number of SH colonies per well (c), the number of cells per colony (d), and the percentage of cells with BrdU+ nucleus per colony (e) are shown. Asterisk indicates statistically significant differences, p < 0.05

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