Fig. 8

Effects of SCF and IL-6 on the growth of SHs transfected with miR-146a-5p. SHs were plated on 12-well plates and cultured for 3 h. After the culture medium was changed, the combinations of miR-146a-5p mimic and cytokine were added to the medium and cultured for 48 h. After that, fresh medium without cytokines was replenished every other day. Panel a shows the photos of typical SH colonies treated with each cytokine. BrdU was added to the culture medium 24 h before fixation, and immunocytochemistry for BrdU was performed 7 days after plating. Scale bars, 100 μm. The total number of SH colonies per well (b) and the number of cells per colony (c) were measured. Asterisk indicates statistically significant differences, p < 0.05